Coding

Part:BBa_K1190002:Design

Designed by: Nisarg Patel   Group: iGEM13_Arizona_State   (2013-08-30)


Listeriolysin O


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 399
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 399
    Illegal NheI site found at 1380
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 399
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Illegal EcoRI site at 399bp. We plan on introducing a silent mutation similar to Warsaw 2009 iGEM to convert the 399G into 399C to remove the EcoRI site.


Source

Listeria monocytogenes genome.

References